FastQCFastQC Report
Tue 27 Sep 2022
211216_A01221_0088_Capture5-Lenti_Capture5-Lenti_MAN-20211203_LentiBarcode_L000_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename211216_A01221_0088_Capture5-Lenti_Capture5-Lenti_MAN-20211203_LentiBarcode_L000_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46041484
Sequences flagged as poor quality0
Sequence length90
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACCACCGAACGCAACGCACGCACAACTACGGGATTGGCGCCGCCGCC41445819.001840600967597No Hit
CCGACCACCGAACGCAACGCACGCATCGTAGTCGTCCCCTGGTACGTTGT34250797.439115124959916No Hit
CCGACCACCGAACGCAACGCACGCACTTAACAGAAATGTTAAACTATAAC30236376.567201439467069No Hit
CCGACCACCGAACGCAACGCACGTTCGTGGGGAACCTGGCGCTAAACCAT16391603.5601806405718808No Hit
CCGACCACCGAACGCAACGCACGACCCCAGGATTCCCCCGGCTCGCCTGC7843401.7035506501050228No Hit
CCGACCACCGAACGCAACGCACGCTGTGTTCTAGGCCCGCCCACCCCAAC7272171.5794821035742461No Hit
CCGACCACCGAACGCAACGCACGCCGGTGCAGCCCAATCCCATCGTCTAC6399421.3899247904346437No Hit
CCGACCACCGAACGCAACGCACGCACGTTCATTCTCCGTCCTCGCGCCCC5333621.1584378991780542No Hit
CCGACCACCGAACGCAACGCACGCAGGGGAGAAGGGAGGGACGCGGCGCT4472200.9713414102812151No Hit
CCGACCACCGAACGCAACGCACCCTCTGACCCTGCCACAACTACTGCAAA3734320.8110772450340654No Hit
CCGACCACCGAACGCAACGCACGCACTACCGCTCGTGTTTGGTCATGACA3651450.7930782595973666No Hit
CCGACCACCGAACGCAACGCACGCTCTGCGGGTTCCTCCCTTCTTCCGAG3637140.7899701929677159No Hit
CCGACCACCGAACGCAACGCACGCACGATCTCACTTCGATCTATACACTG3393240.7369962271415926No Hit
CCGACCACCGAACGCAACGCACGCACACACACACACACACACACACACAC3098390.6729561540631488No Hit
CCGACCACCGAACGCAACGCACGCACACCCCTTCATATGTCAAAATGACA3063920.665469427527575No Hit
CCGACCACCGAACGCAACGCAACCCGGCGTACTAACACAAGCAGTGTCAC2828500.6143372789634669No Hit
CCGACCACCGAACGCAACGCACGGGGAGAGGCTGTGGGAGTGCACTGTGC2525510.548529235069834No Hit
CCGACCACCGAACGCAACGCACGCACGAACATTAGAGGTTGGTTGACCAG2515360.5463247014366435No Hit
CCGACCACCGAACGCAACGCACGATCTCACTTCGATCTATACACTGGAGG2461860.5347047458331273No Hit
CCGACCACCGAACGCAACGCACGCACGTCCAGCACACACGCACATCCAGC2079260.45160577361059867No Hit
CCGACCACCGAACGCAACGCACGCACGTGGCAGGGAAGCCAAGAGCCGGT1973940.4287307507290599No Hit
CCGACCACCGAACGCAACGCACGCACCCGTATCTGCCCTCCTTGCTGGTA1918260.4166373090841295No Hit
CCGACCACCGAACGCAACGCACGCATGCCTTTGTTATAATGGTCAACTGG1858310.4036164429452361No Hit
CCGACCACCGAACGCAACGCACGCACAAAGAATCAACTGCAATTTTTTCC1690270.36711892257860324No Hit
CCGACCACCGAACGCAACGCACGGGCCACAGGCATGTTCCTGCAGTATAC1612320.3501885386665643No Hit
CCGACCACCGAACGCAACGCACGCAGCTGACTTTGTCTTCTCCGCACGAC1602380.3480296160740605No Hit
CCGACCACCGAACGCAACGCACGCGTGAGACTTCTCCGCCGCCTCCGCCG1502110.326251430123321No Hit
CCGACCACCGAACGCAACGCACGCATAACGCGTCTGGATTGGTCAACGTC1448870.31468794533208355No Hit
CCGACCACCGAACGCAACGCACGCACAGTGCCAGCGCGATGGTCAACACT1422710.3090061128351119No Hit
CCGACCACCGAACGCAACGCACGCCGACTCCTCCAGCAACCCTCGGCGCT1418190.30802438948318867No Hit
CCGACCACCGAACGCAACGCACGCCCTGCAAGGGAGGTATCCGTTACAGC1390850.30208626637664415No Hit
CCGACCACCGAACGCAACGCACGCACACACACATCTGTGTAAATAATTAA1311210.2847888221847932No Hit
CCGACCACCGAACGCAACGCACGCATAACGCGTCTGGATTGGTGTACTGG1308010.28409379680290064No Hit
CCGACCACCGAACGCAACGCACGCATGATCTCCCATAATTGGTACACACT1267250.2752409110010442No Hit
CCGACCACCGAACGCAACGCACGCAAGAGACGCGGAGAGATAGGCGATGC1240320.26939183802155464No Hit
CCGACCACCGAACGCAACGCACGCTCACTGCCGAGGAGGCCGAGGAGGAG1237170.26870767241125415No Hit
CCGACCACCGAACGCAACGCACGCATCCCATCCGGCTGGGCCTGGCCCTC1215600.2640227669464347No Hit
CCGACCACCGAACGCAACGCACGTAACAGGAAGCGCCTCGGCCTCAGCGT1184780.257328803737082No Hit
CCGACCACCGAACGCAACGCACGCATGATCTCCCATAATTGGTGTCACAT1155450.25095846172117303No Hit
CCGACCACCGAACGCAACGCACTCACAGTCGCATCATAATCCTCTCTCAA1141170.2478569109544775No Hit
CCGACCACCGAACGCAACGCACGCTACAGTACAAGCTGCTGGAGCCAGTT995270.21616809744881377No Hit
CCGACCACCGAACGCAACGCACGCATCAACGCGTGTCGTTGGTACACCAG991570.2153644743510005No Hit
CCGACCACCGAACGCAACGCACTGCCAGAGCAAAGAGGAAATATTCAGAG986550.21427415328315655No Hit
CCGACCACCGAACGCAACGCACGCACCATTCGGCCTTTATGGTACACGTA964900.20957187218378975No Hit
CCGACCACCGAACGCAACGCACGCATCAACGCGTGTCGTTGGTGTCATGA951470.20665493753415942No Hit
CCGACCACCGAACGCAACGCACGCGCCAGACCTCCAGTAAATAGGCGAGT924440.2007841450114857No Hit
CCGACCACCGAACGCAACGCACCAAGAAGTGAAATAGCCACAGAGCGGGA902090.19592982710982992No Hit
CCGACCACCGAACGCAACGCACCTTTAAGGGCGAGAGCGGTCCGCCGTGC901420.19578430617049614No Hit
CCGACCACCGAACGCAACGCACGCACCATTCGGCCTTTATGGTACTGTGT884600.19213107900692342No Hit
CCGACCACCGAACGCAACGCACCCGCCGCACCCGCCGAGGACGCCGTGGC874890.1900221113637432No Hit
CCGACCACCGAACGCAACGCACGCTGACCCACTACAAGTCAGCTGTCAAA865860.18806083661421513No Hit
CCGACCACCGAACGCAACGCACAACTACGGGATTGGCGCCGCCGCCTGCG864400.18774373128372665No Hit
CCGACCACCGAACGCAACGCACGCTGACATCAAGCAGACCCTGCCGTCAG860800.18696182772909753No Hit
CCGACCACCGAACGCAACGCACGCACACGCACACATCTTACCTCTCATGC854860.18567168686395946No Hit
CCGACCACCGAACGCAACGCACGCACCACGGAGACCAGCAGTGCAGTCAT833470.18102587657687141No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG811830.176325767431823No Hit
CCGACCACCGAACGCAACGCACGCAGACTATCGGTACATTGGTTGGTCAG793960.17244448506481677No Hit
CCGACCACCGAACGCAACGCACGAGCAAGTAACCATTAGAGTAACATTAC787160.1709675561282951No Hit
CCGACCACCGAACGCAACGCACGTTCGTGGGGAACCTGGCGCTAAACCAA784810.17045714686346772No Hit
CCGACCACCGAACGCAACGCACGCAGTTTAGATATACACTGGTCATGACT768930.16700808340582593No Hit
CCGACCACCGAACGCAACGCACGCATGCCTTTGTTATAATGGTTGACACA766700.16652373759281955No Hit
CCGACCACCGAACGCAACGCACCACGACCTTCCCAGGGTGACACCGCCTC765510.16626527502892827No Hit
CCGACCACCGAACGCAACGCACCCTCGGACTGCCCCAAGGCCCCCGCCGC757690.16456680675192834No Hit
CCGACCACCGAACGCAACGCACGCACACACACATACACACAGCCAGGTGT747330.16231666207805118No Hit
CCGACCACCGAACGCAACGCACGCACCCACCATGGCCACAGTTCAGCAGC739640.16064642920719063No Hit
CCGACCACCGAACGCAACGCACGCACTCCCGCACTCCCACGGGAGACTGC736030.1598623536982431No Hit
CCGACCACCGAACGCAACGCACGTGTAACTGTCAGCCGGGCCCTGAGTAA722340.156888948236334No Hit
CCGACCACCGAACGCAACGCACGCAGGAAGTGCATTTTATGGTTGCATGA714280.15513835305569212No Hit
CCGACCACCGAACGCAACGCACGCAATTATTCTGCGCCTTGGTACCAACG700220.1520845852840017No Hit
CCGACCACCGAACGCAACGCACGCTGCCTTTTGAAAGACTCATTGCTTTG682190.14816855164790083No Hit
CCGACCACCGAACGCAACATGAAGGTGCTCCTTGCCGCCGCCCTCATCGC677070.14705651103687276No Hit
CCGACCACCGAACGCAACGCACGTCCATCAGGCCTGGCCTCAGTGGCCAG671960.14594664238016308No Hit
CCGACCACCGAACGCAACGCACGCAGTTTAGATATACACTGGTTGCAGTC668900.14528202435872833No Hit
CCGACCACCGAACGCAACGCACGTCATAGTCCAGCCTCACCTTCCTTAAC648130.14077087523938195No Hit
CCGACCACCGAACGCAACGCACGCACACACGCTGGAGGTTCCAAAACCAC646180.14034734414729116No Hit
CCGACCACCGAACGCAACGCACGCAACAGAAAAATGTACTGGTCATGACC645470.14019313539068376No Hit
CCGACCACCGAACGCAACGCACGTTCATTCTCCGTCCTCGCGCCCCTTTT643260.1397131334863142No Hit
CCGACCACCGAACGCAACCCCTACACCATCTTCCATCAAGCACTGAAAAA617790.13418116583731315No Hit
CCGACCACCGAACGCAACGCACGCGTGAGACTTCTCCGCCGCAGACGCCG607870.13202658715344623No Hit
CCGACCACCGAACGCAACGCACCCGGCGTACTAACACAAGCAGTGTCACC601640.13067345961307414No Hit
CCGACCACCGAACGCAACGCACGCATGTTGACGGGAGTATGGTTGACCAG597660.1298090217943453No Hit
CCGACCACCGAACGCAACGCACGCATGGCGTCGACACGATGGTCAACACC592920.12877951544741695No Hit
CCGACCACCGAACGCAACGCACGCATCACTAAACACCTATGGTTGACCAG566440.12302818041225604No Hit
CCGACCACCGAACGCAACGCACGCAACAGAAAAATGTACTGGTGTCAACC561070.1218618409432676No Hit
CCGACCACCGAACGCAACGCACGCATGCCTTTGTTATAATGGTTGGTCAG543350.11801313789103758No Hit
CCGACCACCGAACGCAACGCACGCTGCCTGGGAACCCTCAGCAAATATTA531550.11545023179530876No Hit
CCGACCACCGAACGCAACGCACGCATGGCGTCGACACGATGGTACACACC531330.11540244880030366No Hit
CCGACCACCGAACGCAACGCACGCGTGGCGCTGCTGCAGTTTGGTGGCCC530820.11529167913006454No Hit
CCGACCACCGAACGCAACGCACGCAGCCGAGCAGCGCCACAGCCGCCGCC527860.11464878065181393No Hit
CCGACCACCGAACGCAACGCACGCACTACCGCTCGTGTTTGGTTGACTGC526110.11426868864609142No Hit
CCGACCACCGAACGCAACGGACTCCCAGGAGCCTCGAGCTCCTCATTTCC525480.11413185552403132No Hit
CCGACCACCGAACGCAACGCACGCACAGTGCCAGCGCGATGGTTGCAGTG519840.11290687328844569No Hit
CCGACCACCGAACGCAACGCACGCTGGGGCCTGTGAGGTGGGGGAGGGAG519650.11286560615639583No Hit
CCGACCACCGAACGCAACGCACGCATAACGCGTCTGGATTGGTTGGTGTC493650.10721852492851883No Hit
CCGACCACCGAACGCAACGCACGCACTAAAGGCGGTCGTTGGTACACTGC492730.1070187051312247No Hit
CCGACCACCGAACGCAACGCACGCAATTAGGATTAATGGTGGTCAACGTA487260.1058306461190521No Hit
CCGACCACCGAACGCAACGCACGCAGATCCTCTACTCCACAGACATCGCC486590.10568512517971836No Hit
CCGACCACCGAACGCAACGCACGCATTCGCGTAGCGGAGTGGTTGTGCAC484160.1051573402803437No Hit
CCGACCACCGAACCCACCCAAACCGATGCCAAATCCAAACCCCAACCACC476260.10344149636879645No Hit
CCGACCACCGAACGCAACGCACGCAATTGCACCCAGTACTGGTTGCATGC475330.10323950461718394No Hit
CCGACCACCGAACGCAACGCACGCACACACAATACAATAATACTAGAGCA461140.10015750143935412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)